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A Molecular Approach to Apoptosis in the Human Heart During Brain Death.

Identificadores del recurso

Pérez López, S...[et al.].A Molecular Approach to Apoptosis in the Human Heart During Brain Death. Transplantation. 2008 ; 86 : 977-982

0041-1337

https://ria.asturias.es/RIA/handle/123456789/9867

Procedència

(Repositorio Institucional de Asturias: RIA)

Fitxa

Títol:: A Molecular Approach to Apoptosis in the Human Heart During Brain Death.
Tema:: Muerte cerebral; Genes de apoptosis; Trasplante de corazón; Donador de órganos.; Publicado
Descripció:: Background. Brain death induces changes in tissues and organs destined for transplant at the cell, molecular, and endocrine level including cell death through apoptosis. This study was designed to examine apoptotic damage in cardiac tissue obtained from brain dead donors. Methods. Fifty tissue specimens from the left ventricles of individual donors were processed to evaluate changes in the expression levels of five genes involved in apoptosis (BAX, BCL2, CASPASE 3, CYTOCHROME C, and FAS) using the real time-polymerase chain reaction technique. Expression levels were quantified by the relative standard method and results normalized to the levels recorded for the endogenous control peptidylprolyl isomerase A. The HIF1 gene was also determined to check for the possibility of hypoxic damage. Control ventricular tissue specimens were obtained from patients undergoing mitral valve replacement. Results. Using a mixed linear model it was determined that the sample type (donor vs. control patient) significantly affected (P 0.0001) expression levels of the genes examined reflected by their Ct values. Three of the genes (BAX, CASPASE 3, and FAS) showed significantly higher (Student’s t test, P 0.05) expression levels (4.89-, 7.85-, and 12.14-fold endogenous control values, respectively) in donors compared with control patients (2.31-, 2.64-, and 3.57-fold endogenous control values, respectively) indicating the activation of apoptosis during brain death. Conclusion. Our findings suggest the possibility of using antiapoptosis agents to prevent cardiac injury and improve posttransplant behavior.
Font:: 977;982
Idioma:: English
Relació:: Transplantation; 86; Sí, esta versión ha sido citada
Autor/Productor:: Pérez López, Silvia; Vázquez Moreno, Natalia; Escudero Augusto, Dolores; Astudillo González, Aurora; Alvarez Menéndez, Francisco; Goyache Goñi, Félix; Otero Hernández, Jesús
Editor:: Lippincott, Williams & Wilkins
Drets:: http://creativecommons.org/licenses/by-nc-nd/3.0/deed.es
Data:: 2018-03-27T11:29:42Z; 2008
Tipo de recurso:: article
Format:: application/pdf

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2. <dc:creator>Pérez López, Silvia</dc:creator>
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