Identificadores del recurso
Procedència
(Repositorio Institucional de Asturias: RIA)Fitxa
- Títol:
- A mutation in Flavobacterium psychrophilum tlpB inhibits gliding motility and induces biofilm formation
- Tema:
- Publicado
- Descripció:
- Flavobacterium psychrophilum is a psychrotrophic, fish-pathogenic bacterium belonging to the Cytophaga-Flavobacterium-Bacteroides group. Tn4351-induced mutants deficient in gliding motility, growth on iron-depleted media, and extracellular proteolytic activity were isolated. Some of these mutants were affected in only one of these characteristics, whereas others had defects in two or more. FP523, a mutant deficient in all of these properties, was studied further. FP523 had a Tn4351 insertion in tlpB (thiol oxidoreductase-like protein gene), which encodes a 41.4-kDa protein whose sequence does not exhibit high levels of similar to the sequences of proteins having known functions. TlpB has two domains; the N-terminal domains has five transmembrane regions, whereas the C-terminal domains has the Cys-X-X-Cys motif and other conserved motifs characteristic of thiol:disulfide oxidoreductases. Quantitative analysis of the thiol groups of periplasmic proteins revealed that TlpB is required for reduction of these groups. The tlpB gene is part of the fpt (F. psychrophilum thiol oxidoreductase) operon that contains two other genes, tlpA and tpiA, which encode a thiol:disulfide oxidoreductase and a triosephosphate isomerase, respectively. FP523 exhibited enhanced biofilm formation and decreased virulence and cytotoxicity. Complementation with the tlpB loci restored the wild-type phenotype. Gliding motility and biofilm formation appear to be antagonistic properties, which are both affected by TlpB.
- Font:
- 4044;4053
- Idioma:
- English
- Relació:
- Applied and Environmental Microbiology
- 72
- 6
- Autor/Productor:
- Álvarez, B.
- Secades, P.
- Prieto Martín, Miguel
- McBride, M. J.
- Guijarro, J. A.
- Editor:
- American Society for Microbiology
- Drets:
- http://creativecommons.org/licenses/by-nc-sa/3.0/deed.es
- Data:
- 2012-11-27T11:00:29Z
- 2006-07
- Tipo de recurso:
- article
- Format:
- application/pdf
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