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??-catenin fluctuates in mouse ESCs and is essential for Nanog-mediated reprogramming of somatic cells to pluripotency

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Títol:: ??-catenin fluctuates in mouse ESCs and is essential for Nanog-mediated reprogramming of somatic cells to pluripotency
Descripció:: The Wnt/??-catenin pathway and Nanog are key regulators of embryonic stem cell (ESC) pluripotency and the reprogramming of somatic cells. Here, we demonstrate that the repression of Dkk1 by Nanog, which leads indirectly to ??-catenin activation, is essential for reprogramming after fusion of ESCs overexpressing Nanog. In addition, ??-catenin is necessary in Nanog-dependent conversion of preinduced pluripotent stem cells (pre-iPSCs) into iPSCs. The activation of ??-catenin by Nanog causes fluctuations of ??-catenin in ESCs cultured in serum plus leukemia inhibitory factor (serum+LIF) medium, in which protein levels of key pluripotency factors are heterogeneous. In 2i+LIF medium, which favors propagation of ESCs in a ground state of pluripotency with many pluripotency genes losing mosaic expression, we show Nanog-independent ??-catenin fluctuations. Overall, we demonstrate Nanog and ??-catenin cooperation in establishing naive pluripotency during the reprogramming process and their correlated heterogeneity in ESCs primed toward differentiation.
Llengua:: eng
Relació:: info:eu-repo/grantAgreement/EC/ERC/242630; info:eu-repo/grantAgreement/ES/3PN/SAF2011-28580
Autor/Productor:: Marucci, Lucia; Pedone, Elisa, 1985-; Vicino, Umberto di; Sanuy-Escribano, Blanca; Isalan, Mark; Cosma, Maria Pia
Editor:: Elsevier
Drets:: http://creativecommons.org/licenses/by-nc-nd/3.0/; info:eu-repo/semantics/openAccess; ?? Elsevier http://dx.doi.org/10.1016/j.celrep.2014.08.011
Data:: 2014
Tipus de recurs:: info:eu-repo/semantics/article; info:eu-repo/semantics/publishedVersion
Identificador del recurs:: Marucci L, Pedone E, Di Vicino U, Sanuy-Escribano B, Isalan M, Cosma MP. ??-catenin fluctuates in mouse ESCs and is essential for Nanog-mediated reprogramming of somatic cells to pluripotency. Cell Rep. 2014 Sep 25;8(6):1686-96. DOI: 10.1016/j.celrep.2014.08.011; 2211-1247; http://hdl.handle.net/10230/23505; http://dx.doi.org/10.1016/j.celrep.2014.08.011

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