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3D Printed Tacrolimus Rectal Formulations Ameliorate Colitis in an Experimental Animal Model of Inflammatory Bowel Disease.

Identificadores del recurso

2227-9059

https://www.ncbi.nlm.nih.gov/pubmed/33276641

http://hdl.handle.net/20.500.11940/16389

10.3390/biomedicines8120563

33276641

36924

Procedencia

(RUNA repositorio institucional de la Consellería de Sanidade - Sergas)

Ficha

Título:: 3D Printed Tacrolimus Rectal Formulations Ameliorate Colitis in an Experimental Animal Model of Inflammatory Bowel Disease.
Tema:: CHUS; CHUO; IDIS
Descrición:: The aim of this study was to fabricate novel self-supporting tacrolimus suppositories using semisolid extrusion 3-dimensional printing (3DP) and to investigate their efficacy in an experimental model of inflammatory bowel disease. Blends of Gelucire 44/14 and coconut oil were employed as lipid excipients to obtain suppository formulations with self-emulsifying properties, which were then tested in a TNBS (2,4,6-trinitrobenzenesulfonic acid) induced rat colitis model. Disease activity was monitored using PET/CT medical imaging; maximum standardized uptake values (SUVmax), a measure of tissue radiotracer accumulation rate, together with body weight changes and histological assessments, were used as inflammatory indices to monitor treatment efficacy. Following tacrolimus treatment, a significant reduction in SUVmax was observed on days 7 and 10 in the rat colon sections compared to non-treated animals. Histological analysis using Nancy index confirmed disease remission. Moreover, statistical analysis showed a positive correlation (R(2) = 71.48%) between SUVmax values and weight changes over time. Overall, this study demonstrates the effectiveness of 3D printed tacrolimus suppositories to ameliorate colitis and highlights the utility of non-invasive PET/CT imaging to evaluate new therapies in the preclinical area.
Autor/Productor:: Seoane-Viaño, Iria; Gómez Lado, Noemí; Lazare Iglesias, Hector; García Otero, Xurxo; Antúnez López, José Ramón; Ruibal Morell, Alvaro; Varela Correa, Juan Jesús; Aguiar Fernández, Pablo; Basit, Abdul W; Otero-Espinar, Francisco J; González Barcia, Miguel; Goyanes, Alvaro; Luzardo-Álvarez, Asteria; Fernández Ferreiro, Anxo
Dereitos:: Atribución 4.0 Internacional; http://creativecommons.org/licenses/by/4.0/; openAccess
Data:: 2022-03-23T08:56:17Z; 2020
Tipo de recurso:: Journal Article

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