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<oai_dc:dc schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
<dc:title>Evaluation of Different Thawing Protocols on Iberian Boar Sperm Preserved for 10 Years at Different Liquid Nitrogen Levels</dc:title>
<dc:creator>Álvarez-Rodríguez, Manuel</dc:creator>
<dc:creator>Tomás-Almenar, Cristina</dc:creator>
<dc:creator>Nieto-Cristóbal, Helena</dc:creator>
<dc:creator>de Mercado, Eduardo</dc:creator>
<dc:description>The conservation of genetic resources in pig breeds, notably the Iberian pig, is crucial for genetic improvement and sustainable production. Prolonged storage in liquid nitrogen (LN<sub>2</sub>) is recognized for preserving genetic diversity, but potential adverse effects on seminal quality remain debated. This study aims to assess the impact of ten years of storage at different LN<sub>2</sub> levels and to optimize thawing protocols for Iberian pig sperm. Sperm samples from 53 boars were cryopreserved and stored at varying LN<sub>2</sub> levels and, a decade later, the samples were thawed at 37 °C for 20 s or at 70 °C for 8 s. Sperm motility, membrane integrity, acrosome status, and DNA fragmentation were evaluated in year 0 and year 10. Overall, no significant differences were observed in post-thaw sperm quality between storage levels in year 0 or year 10. But thawing at 70 °C 8 s showed significant improvements, particularly in samples that were always stored in LN<sub>2</sub>, in all analyzed parameters except fragmentation, which was not affected by cryostorage. This study suggests that the long-term preservation of Iberian pig sperm does not affect quality over time, regardless of whether the samples were fully submerged in LN<sub>2</sub>. Furthermore, it is determined that thawing at 70 °C for 8 s maximizes post-thaw sperm quality, especially in those samples stored constantly submerged in LN<sub>2</sub>.</dc:description>
<dc:date>2024-05-04T10:02:57Z</dc:date>
<dc:date>2024-05-04T10:02:57Z</dc:date>
<dc:date>2024-03-15</dc:date>
<dc:date>2024-05-04T10:02:57Z</dc:date>
<dc:type>artículo</dc:type>
<dc:identifier>doi: 10.3390/ani14060914</dc:identifier>
<dc:identifier>Animals 14 (6): 914 (2024)</dc:identifier>
<dc:identifier>http://hdl.handle.net/10261/355844</dc:identifier>
<dc:rights>open</dc:rights>
<dc:publisher>Multidisciplinary Digital Publishing Institute</dc:publisher>
</oai_dc:dc>