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<oai_dc:dc schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
<dc:title>'Artilysation' of endolysin λSa2lys strongly improves its enzymatic and antibacterial activity against streptococci</dc:title>
<dc:creator>Rodríguez, Lorena</dc:creator>
<dc:creator>Gutiérrez, Diana</dc:creator>
<dc:creator>Martínez Fernández, Beatriz</dc:creator>
<dc:creator>Rodríguez González, Ana</dc:creator>
<dc:creator>García Suárez, María Pilar</dc:creator>
<dc:contributor>Research Foundation - Flanders</dc:contributor>
<dc:contributor>Principado de Asturias</dc:contributor>
<dc:contributor>European Commission</dc:contributor>
<dc:contributor>Ministerio de Ciencia e Innovación (España)</dc:contributor>
<dc:subject>Antibiotics</dc:subject>
<dc:subject>Bacteriophages</dc:subject>
<dc:description>Endolysins constitute a promising class of antibacterials against Gram-positive bacteria. Recently, endolysins have been engineered with selected peptides to obtain a new generation of lytic proteins, Artilysins, with specific activity against Gram-negative bacteria. Here, we demonstrate that artilysation can also be used to enhance the antibacterial activity of endolysins against Gram-positive bacteria and to reduce the dependence on external conditions. Art-240, a chimeric protein of the anti-streptococcal endolysin λSa2lys and the polycationic peptide PCNP, shows a similar species specificity as the parental endolysin, but the bactericidal activity against streptococci increases and is less affected by elevated NaCl concentrations and pH variations. Time-kill experiments and time-lapse microscopy demonstrate that the killing rate of Art-240 is approximately two-fold higher compared to wildtype endolysin λSa2lys, with a reduction in viable bacteria of 3 log units after 10 min. In addition, lower doses of Art-240 are required to achieve the same bactericidal effect.</dc:description>
<dc:description>This research study was supported by grants AGL2012-40194-C02-01 (Ministry of Science and Innovation, Spain), FEDER founds and GRUPIN14-139 (Program of Science, Technology and Innovation 2013–2017, Principado de Asturias, Spain), bacteriophage network FAGOMA and research grant 1.5.171.15N of the Research Foundation – Flanders (FWO). DG was a fellow of the Ministry of Science and Innovation, Spain. LR-R was a FWO Pegasus Marie Curie Fellow. PG, BM, RL and AR are members of the FWO Vlaanderen funded “Phagebiotics” research community (WO.016.14).</dc:description>
<dc:description>Peer Reviewed</dc:description>
<dc:date>2017-02-01T08:29:01Z</dc:date>
<dc:date>2017-02-01T08:29:01Z</dc:date>
<dc:date>2016-10-26</dc:date>
<dc:date>2017-02-01T08:29:01Z</dc:date>
<dc:type>artículo</dc:type>
<dc:type>http://purl.org/coar/resource_type/c_6501</dc:type>
<dc:identifier>issn: 2045-2322</dc:identifier>
<dc:identifier>Scientific Reports 6: 35382 (2016)</dc:identifier>
<dc:identifier>http://hdl.handle.net/10261/143246</dc:identifier>
<dc:identifier>10.1038/srep35382</dc:identifier>
<dc:identifier>http://dx.doi.org/10.13039/501100000780</dc:identifier>
<dc:identifier>http://dx.doi.org/10.13039/501100004837</dc:identifier>
<dc:identifier>http://dx.doi.org/10.13039/501100003130</dc:identifier>
<dc:identifier>http://dx.doi.org/10.13039/100011941</dc:identifier>
<dc:identifier>27775093</dc:identifier>
<dc:relation>Publisher's version</dc:relation>
<dc:relation>https://doi.org/10.1038/srep35382</dc:relation>
<dc:relation>Sí</dc:relation>
<dc:rights>open</dc:rights>
<dc:publisher>Nature Publishing Group</dc:publisher>
</oai_dc:dc>