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9-cis-retinoic acid during in vitro maturation improves development of the bovine oocyte and increases midkine but not IGF-I expression in cumulus-granulosa cells

Identificadores del recurso

http://ria.asturias.es/RIA/bitstream/123456789/2088/1/9-cis-retinoic.pdf

Jatorria

(Repositorio Institucional de Asturias : RIA)

Fitxa

Izenburu:: 9-cis-retinoic acid during in vitro maturation improves development of the bovine oocyte and increases midkine but not IGF-I expression in cumulus-granulosa cells
Tema:: Embryo; Ethanol; Viability; Cytoplasmic granule; Publicado
Deskribapen:: The isomer 9-cis of retinoic acid (9-cis-RA) exerts a beneficial effect on bovine in vitro development when added to in vitro maturation (IVM) culture. In the present work, 9-cis-RA 5 nM was found to be stimulatory as opposed to 500 nM (toxic). Cumulus-oocyte complexes (COCs) were treated with the found physiological dose 9-cis-RA 5 nM, and the next determinations performed: (1) relative expression of midkine (MK) and IGF-I, by reverse transcriptase-polymerase chain reaction (RT-PCR), in cumulus-granulosa cells detached from oocytes; (2) cytoplasmic granular migration, by labeling of oocytes with fluoroscein isothiocyanate lectins; and (3) in vitro survival of blastocysts after vitrification and warming. Gene expression of MK was enhanced by 9-cis-RA, but not by 1% ethanol (vehicle). However, we did not detect IGF-I expression, both in dependence on or in the absence of 9-cis-RA acting on cumulus-granulosa cells. The ability of vitrified blastocysts to survive in vitro was not improved by 9-cis-RA. Nevertheless, since only blastocysts obtained from oocytes matured with serum survived, more factors should be considered when evaluating cryopreservation survival. The complete granular migration observed in oocytes matured with 9-cis-RA anticipates the gain in developmental competence of the oocyte, being MK probably involved in this beneficial effect.
Iturri:: Gómez, Enrique; Royo, Luis-José; Duque, Paloma; Carneiro, Gustavo; Hidalgo, Carlos; Goyache, Félix [et. al]. 9-cis-retinoic acid during in vitro maturation improves development of the bovine oocyte and increases midkine but not IGF-I expression in cumulus-granulosa cells. Molecular Reproduction and Development. 2003; 66 (3): 247-255.; ISSN: 1040-452X; 247;255
Idioma:: English
Harreman:: Molecular Reproduction and Development; 66; 3
Autor/Productor:: Gómez, Enrique; Royo, L.J. (Luis); Duque, Paloma; Carneiro, Gustavo; Hidalgo, C.O. (Carlos); Goyache, Félix; Lorenzo, P.L. (Pedro); Álvarez, Isabel; Facal, Nieves; Díez, Carmen
Argitaratzaile:: WILEY-LISS, INC
Eskubideak:: http://creativecommons.org/licenses/by-nc-sa/3.0/deed.es
Data:: 2012-11-07
Tipo de recurso:: other
Formatu:: application/pdf

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