3D printed carboxymethyl cellulose scaffolds for autologous growth factors delivery in wound healing
Díaz Gómez, Luis Antonio
González Prada, Iago
Millán Lence, Rosendo
Silva Candal, Andrés da
Bugallo Casal, Ana
Campos Pérez, Francisco
Concheiro Nine, Ángel Joaquín
Álvarez Lorenzo, Carmen Isabel
Universidade de Santiago de Compostela. Departamento de Farmacia e Tecnoloxía Farmacéutica
Universidade de Santiago de Compostela. Departamento de Farmacoloxía, Farmacia e Tecnoloxía Farmacéutica
Carboxymethyl cellulose
3D printing
Rheological properties
MicroCT
Platelet rich plasma
Wound healing
Cell mobility
Angiogenesis
This work aims to use carboxymethyl cellulose (CMC) as main structural and functional component of 3D printed scaffolds for healing of diabetic wounds. Differently from previous inks involving small contents in CMC, herein sterile (steam-heated) concentrated CMC solely dispersions (10–20%w/v) were screened regarding printability and fidelity properties. CMC (15%w/v)-citric acid inks showed excellent self-healing rheological properties and stability during storage. CMC scaffolds loaded with platelet rich plasma (PRP) sustained the release of relevant growth factors. CMC scaffolds both with and without PRP promoted angiogenesis in ovo, stem cell migration in vitro, and wound healing in a diabetic model in vivo. Transparent CMC scaffolds allowed direct monitoring of bilateral full-thickness wounds created in rat dorsum. CMC scaffolds facilitated re-epithelialization, granulation, and angiogenesis in full-thickness skin defects, and the performance was improved when combined with PRP. Overall, CMC is pointed out as outstanding component of active dressings for diabetic wounds
SI
2022-01-31T09:11:52Z
2022-01-31T09:11:52Z
2022
info:eu-repo/semantics/article
info:eu-repo/semantics/draft
Carbohydrate Polymers 278 (2022) 118924
http://hdl.handle.net/10347/27437
10.1016/j.carbpol.2021.118924
0144-8617
eng
https://doi.org/10.1016/j.carbpol.2021.118924
© 2022 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)2
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
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Elsevier