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β-carotene and Bacillus thuringiensis insecticidal protein differentially modulate feeding behaviour, mortality and physiology of European corn borer (Ostrinia nubilalis)

Identificadores del recurso

1932-6203

http://hdl.handle.net/10459.1/70709

https://doi.org/10.1371/journal.pone.0246696

Procedencia

(Repositori Obert UdL)

Ficha

Título:: β-carotene and Bacillus thuringiensis insecticidal protein differentially modulate feeding behaviour, mortality and physiology of European corn borer (Ostrinia nubilalis)
Descripción:: Maize with enhanced β-carotene production was engineered to counteract pervasive vitamin A deficiency in developing countries. Second-generation biofortified crops are being developed with additional traits that confer pest resistance. These include crops that can produce Bacillus thuringiensis Berliner (Bt) insecticidal proteins. Currently, it is unknown whether β-carotene can confer fitness benefits through to insect pests, specifically through altering Ostrinia nubilalis foraging behaviour or development in the presence of Bt insecticidal toxin. Therefore the effects of dietary β-carotene plus Bt insecticidal protein on feeding behaviour, mortality, and physiology in early and late instars of O. nubilalis larvae were investigated. The results of two-choice experiments showed that irrespective of β-carotene presence, at day five 68%-90% of neonates and 69%-77% of fifth-instar larvae avoided diets with Cry1A protein. Over 65% of neonate larvae preferred to feed on diets with β-carotene alone compared to 39% of fifth-instar larvae. Higher mortality (65%-97%) in neonates fed diets supplemented with β-carotene alone and in combination with Bt protein was found, whereas <36% mortality was observed when fed diets without supplemented β-carotene or Bt protein. Diets with both β-carotene and Bt protein extended 25 days the larval developmental duration from neonate to fifth instar (compared to Bt diets) but did not impair larval or pupal weight. Juvenile hormone and 20-hydroxyecdysone regulate insect development and their levels were at least 3-fold higher in larvae fed diets with β-carotene for 3 days. Overall, these results suggest that the effects of β-carotene and Bt protein on O. nubilalis is dependent on larval developmental stage. This study is one of the first that provides insight on how the interaction of novel traits may modulate crop susceptibility to insect pests. This understanding will in turn inform the development of crop protection strategies with greater efficacy.; This work was partially supported through an Agrotecnio Fellowship and the AGL2017-84127-R project from the Spanish R&D Agency (Comisión Interministerial de Ciencia y Tecnología).
Idioma:: English
Relación:: info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-84127-R/ES/MANEJO DE CULTIVOS EXTENSIVOS E INTERACCIONES CON EL PAISAJE PARA EL CONTROL DE PLAGAS/; Reproducció del document publicat a: https://doi.org/10.1371/journal.pone.0246696; PLoS ONE, 2021, vol. 16, núm. 2, e0246696
Autor/Productor:: Girón-Calva, Patricia Sarai; López Alonso, Carmen; Albacete, Alfonso; Albajes Garcia, Ramon; Christou, Paul; Eizaguirre Altuna, Matilde
Editor:: Public Library of Science
Derechos:: cc-by (c) Girón-Calva et al., 2021; http://creativecommons.org/licenses/by/4.0/; info:eu-repo/semantics/openAccess
Fecha:: 2021-03-10T08:35:55Z; 2021-02-16
Tipo de recurso:: info:eu-repo/semantics/article; info:eu-repo/semantics/publishedVersion

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  16. <dc:identifier>https://doi.org/10.1371/journal.pone.0246696</dc:identifier>
  17. <dc:language>eng</dc:language>
  18. <dc:relation>Reproducció del document publicat a: https://doi.org/10.1371/journal.pone.0246696</dc:relation>
  19. <dc:relation>PLoS ONE, 2021, vol. 16, núm. 2, e0246696</dc:relation>
  20. <dc:relation>info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-84127-R/ES/MANEJO DE CULTIVOS EXTENSIVOS E INTERACCIONES CON EL PAISAJE PARA EL CONTROL DE PLAGAS/</dc:relation>
  21. <dc:rights>http://creativecommons.org/licenses/by/4.0/</dc:rights>
  22. <dc:rights>info:eu-repo/semantics/openAccess</dc:rights>
  23. <dc:rights>cc-by (c) Girón-Calva et al., 2021</dc:rights>
  24. <dc:publisher>Public Library of Science</dc:publisher>
  </ow:Publication>
</rdf:RDF>