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3D Printed Tacrolimus Rectal Formulations Ameliorate Colitis in an Experimental Animal Model of Inflammatory Bowel Disease

Identificadores del recurso

Seoane-Viaño, I.; Gómez-Lado, N.; Lázare-Iglesias, H.; García-Otero, X.; Antúnez-López, J.R.; Ruibal, Á.; Varela-Correa, J.J.; Aguiar, P.; Basit, A.W.; Otero-Espinar, F.J.; González-Barcia, M.; Goyanes, A.; Luzardo-Álvarez, A.; Fernández-Ferreiro, A. 3D Printed Tacrolimus Rectal Formulations Ameliorate Colitis in an Experimental Animal Model of Inflammatory Bowel Disease. Biomedicines 2020, 8, 563

http://hdl.handle.net/10347/24017

10.3390/biomedicines8120563

2227-9059

Procedencia

(Minerva. Repositorio Institucional da Universidade de Santiago de Compostela)

Ficha

Título:: 3D Printed Tacrolimus Rectal Formulations Ameliorate Colitis in an Experimental Animal Model of Inflammatory Bowel Disease
Tema:: Three-dimensional printing; PET/CT imaging; Rectal drug delivery; Ulcerative colitis; TNBS rat model; M3dimaker; 3D printed drug products; Personalized medicines and pharmaceuticals
Descripción:: The aim of this study was to fabricate novel self-supporting tacrolimus suppositories using semisolid extrusion 3-dimensional printing (3DP) and to investigate their efficacy in an experimental model of inflammatory bowel disease. Blends of Gelucire 44/14 and coconut oil were employed as lipid excipients to obtain suppository formulations with self-emulsifying properties, which were then tested in a TNBS (2,4,6-trinitrobenzenesulfonic acid) induced rat colitis model. Disease activity was monitored using PET/CT medical imaging; maximum standardized uptake values (SUVmax), a measure of tissue radiotracer accumulation rate, together with body weight changes and histological assessments, were used as inflammatory indices to monitor treatment efficacy. Following tacrolimus treatment, a significant reduction in SUVmax was observed on days 7 and 10 in the rat colon sections compared to non-treated animals. Histological analysis using Nancy index confirmed disease remission. Moreover, statistical analysis showed a positive correlation (R2 = 71.48%) between SUVmax values and weight changes over time. Overall, this study demonstrates the effectiveness of 3D printed tacrolimus suppositories to ameliorate colitis and highlights the utility of non-invasive PET/CT imaging to evaluate new therapies in the preclinical area; This research was funded by Xunta de Galicia grant number GRC2013/015 and GPC2017/015. A.F.-F. acknowledges the support of Instituto de Salud Carlos III (Juan Rodés research grant JR18/00014). P.A. acknowledges the support of RYC-2015/17430 (Ramón y Cajal). X.G.-O. acknowledges the financial support of the IDIS (Health Research Institute of Santiago de Compostela); SI
Idioma:: English
Relación:: https://doi.org/10.3390/biomedicines8120563
Autor/Productor:: Seoane Viaño, Iria; Gómez Lado, Noemí; Lázare Iglesias, Héctor; García Otero, Xurxo; Antúnez López, José Ramón; Ruibal Morell, Álvaro; Varela Correa, Juan Jesús; Aguiar Fernández, Pablo; Basit, Abdul W.; Otero Espinar, Francisco Javier; González Barcia, Miguel; Goyanes Goyanes, Álvaro; Luzardo Álvarez, Asteria María; Fernández Ferreiro, Anxo
Editor:: MDPI
Otros colaboradores/productores:: Universidade de Santiago de Compostela. Departamento de Farmacoloxía, Farmacia e Tecnoloxía Farmacéutica
Derechos:: © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/); Atribución 4.0 Internacional; http://creativecommons.org/licenses/by/4.0/; open access
Fecha:: 2020-12-16T12:37:53Z; 2020
Tipo de recurso:: journal article; VoR

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1. <dc:title>3D Printed Tacrolimus Rectal Formulations Ameliorate Colitis in an Experimental Animal Model of Inflammatory Bowel Disease</dc:title>
2. <dc:creator>Seoane Viaño, Iria</dc:creator>
3. <dc:creator>Gómez Lado, Noemí</dc:creator>
4. <dc:creator>Lázare Iglesias, Héctor</dc:creator>
5. <dc:creator>García Otero, Xurxo</dc:creator>
6. <dc:creator>Antúnez López, José Ramón</dc:creator>
7. <dc:creator>Ruibal Morell, Álvaro</dc:creator>
8. <dc:creator>Varela Correa, Juan Jesús</dc:creator>
9. <dc:creator>Aguiar Fernández, Pablo</dc:creator>
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11. <dc:creator>Otero Espinar, Francisco Javier</dc:creator>
12. <dc:creator>González Barcia, Miguel</dc:creator>
13. <dc:creator>Goyanes Goyanes, Álvaro</dc:creator>
14. <dc:creator>Luzardo Álvarez, Asteria María</dc:creator>
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16. <dc:contributor>Universidade de Santiago de Compostela. Departamento de Farmacoloxía, Farmacia e Tecnoloxía Farmacéutica</dc:contributor>
17. <dc:subject>Three-dimensional printing</dc:subject>
18. <dc:subject>PET/CT imaging</dc:subject>
19. <dc:subject>Rectal drug delivery</dc:subject>
20. <dc:subject>Ulcerative colitis</dc:subject>
21. <dc:subject>TNBS rat model</dc:subject>
22. <dc:subject>M3dimaker</dc:subject>
23. <dc:subject>3D printed drug products</dc:subject>
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25. <dc:description>The aim of this study was to fabricate novel self-supporting tacrolimus suppositories using semisolid extrusion 3-dimensional printing (3DP) and to investigate their efficacy in an experimental model of inflammatory bowel disease. Blends of Gelucire 44/14 and coconut oil were employed as lipid excipients to obtain suppository formulations with self-emulsifying properties, which were then tested in a TNBS (2,4,6-trinitrobenzenesulfonic acid) induced rat colitis model. Disease activity was monitored using PET/CT medical imaging; maximum standardized uptake values (SUVmax), a measure of tissue radiotracer accumulation rate, together with body weight changes and histological assessments, were used as inflammatory indices to monitor treatment efficacy. Following tacrolimus treatment, a significant reduction in SUVmax was observed on days 7 and 10 in the rat colon sections compared to non-treated animals. Histological analysis using Nancy index confirmed disease remission. Moreover, statistical analysis showed a positive correlation (R2 = 71.48%) between SUVmax values and weight changes over time. Overall, this study demonstrates the effectiveness of 3D printed tacrolimus suppositories to ameliorate colitis and highlights the utility of non-invasive PET/CT imaging to evaluate new therapies in the preclinical area</dc:description>
26. <dc:description>This research was funded by Xunta de Galicia grant number GRC2013/015 and GPC2017/015. A.F.-F. acknowledges the support of Instituto de Salud Carlos III (Juan Rodés research grant JR18/00014). P.A. acknowledges the support of RYC-2015/17430 (Ramón y Cajal). X.G.-O. acknowledges the financial support of the IDIS (Health Research Institute of Santiago de Compostela)</dc:description>
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28. <dc:date>2020-12-16T12:37:53Z</dc:date>
29. <dc:date>2020-12-16T12:37:53Z</dc:date>
30. <dc:date>2020</dc:date>
31. <dc:type>journal article</dc:type>
32. <dc:type>VoR</dc:type>
33. <dc:identifier>Seoane-Viaño, I.; Gómez-Lado, N.; Lázare-Iglesias, H.; García-Otero, X.; Antúnez-López, J.R.; Ruibal, Á.; Varela-Correa, J.J.; Aguiar, P.; Basit, A.W.; Otero-Espinar, F.J.; González-Barcia, M.; Goyanes, A.; Luzardo-Álvarez, A.; Fernández-Ferreiro, A. 3D Printed Tacrolimus Rectal Formulations Ameliorate Colitis in an Experimental Animal Model of Inflammatory Bowel Disease. Biomedicines 2020, 8, 563</dc:identifier>
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37. <dc:language>eng</dc:language>
38. <dc:relation>https://doi.org/10.3390/biomedicines8120563</dc:relation>
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40. <dc:rights>Atribución 4.0 Internacional</dc:rights>
41. <dc:rights>http://creativecommons.org/licenses/by/4.0/</dc:rights>
42. <dc:rights>open access</dc:rights>
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