Hispana. Acceso en línea al Patrimonio Cultural Digital Español

Está en:  › Datos de registro

Daylight photodynamic therapy using methylene blue to treat sheep with dermatophytosis caused by Arthroderma vanbreuseghemii

Identificadores del recurso

http://zaguan.unizar.es/record/70912

10.1016/j.smallrumres.2017.03.011

Procedencia

(Repositorio Institucional Zaguán)

Ficha

Título:: Daylight photodynamic therapy using methylene blue to treat sheep with dermatophytosis caused by Arthroderma vanbreuseghemii
Descripción:: Arthroderma vanbreuseghemii has been identified molecularly as the causative agent of dermatophytosis in a flock of sheep. It is necessary to explore new treatment alternatives because antifungals are not approved for use on small ruminant animals in the European Union. Antimicrobial photodynamic therapy (aPDT) has been shown to be effective for the treatment of dermatophytosis in humans. It is based on the application of a photosensitizer such as methylene blue (MB) that is activated by visible light to generate reactive oxygen species that are cytotoxic to cells. The use of daylight to perform aPDT (aDL-PDT) avoids the requirement of specific equipment because it uses sunlight to activate the photosensitizer. The aim of our study is to determine the efficacy of aDL-PDT using a 1% MB solution to treat dermatophytosis caused by A. vanbreuseghemii in ewes. Two different topical protocols (1% MB solution spray applications once or twice a week) were assayed in two groups of five infected animals. Twenty-five infected sheep were untreated. All the sheep were exposed to sunlight every day for an approximate duration of 10 h for a total of four weeks. At the end of the study, all the animals treated with aDL-PDT showed the same clinical response to both protocols. In contrast, the animals exposed only to sunlight required an additional two to four weeks before their infections resolved. Conclusion: aDL-PDT with 1% MB solution demonstrates efficacy, safety and efficiency in the treatment of dermatophytosis in sheep.
Idioma:: English
Relación:: info:eu-repo/grantAgreement/ES/MICINN/CTQ2013-48767-C3-2-R
Autor/Productor:: Pérez-Laguna, V.; Rezusta, A.; Ramos, J. J.; Ferrer, L. M.; Gené, J.; Revillo, M. J.; Gilaberte, Y.
Derechos:: by-nc-nd; http://creativecommons.org/licenses/by-nc-nd/3.0/es/
Fecha:: 2017
Tipo de recurso:: info:eu-repo/semantics/conferenceObject; info:eu-repo/semantics/acceptedVersion
Formato:: application/pdf

oai_dc

Descargar XML

<?xml version="1.0" encoding="UTF-8" ?>

<oai_dc:dc schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
1. <dc:creator>Pérez-Laguna, V.</dc:creator>
2. <dc:creator>Rezusta, A.</dc:creator>
3. <dc:creator>Ramos, J. J.</dc:creator>
4. <dc:creator>Ferrer, L. M.</dc:creator>
5. <dc:creator>Gené, J.</dc:creator>
6. <dc:creator>Revillo, M. J.</dc:creator>
7. <dc:creator>Gilaberte, Y.</dc:creator>
8. <dc:title>Daylight photodynamic therapy using methylene blue to treat sheep with dermatophytosis caused by Arthroderma vanbreuseghemii</dc:title>
9. <dc:identifier>http://zaguan.unizar.es/record/70912</dc:identifier>
10. <dc:publisher />
11. <dc:description>Arthroderma vanbreuseghemii has been identified molecularly as the causative agent of dermatophytosis in a flock of sheep. It is necessary to explore new treatment alternatives because antifungals are not approved for use on small ruminant animals in the European Union. Antimicrobial photodynamic therapy (aPDT) has been shown to be effective for the treatment of dermatophytosis in humans. It is based on the application of a photosensitizer such as methylene blue (MB) that is activated by visible light to generate reactive oxygen species that are cytotoxic to cells. The use of daylight to perform aPDT (aDL-PDT) avoids the requirement of specific equipment because it uses sunlight to activate the photosensitizer. The aim of our study is to determine the efficacy of aDL-PDT using a 1% MB solution to treat dermatophytosis caused by A. vanbreuseghemii in ewes. Two different topical protocols (1% MB solution spray applications once or twice a week) were assayed in two groups of five infected animals. Twenty-five infected sheep were untreated. All the sheep were exposed to sunlight every day for an approximate duration of 10 h for a total of four weeks. At the end of the study, all the animals treated with aDL-PDT showed the same clinical response to both protocols. In contrast, the animals exposed only to sunlight required an additional two to four weeks before their infections resolved. Conclusion: aDL-PDT with 1% MB solution demonstrates efficacy, safety and efficiency in the treatment of dermatophytosis in sheep.</dc:description>
12. <dc:format>application/pdf</dc:format>
13. <dc:language>eng</dc:language>
14. <dc:date>2017</dc:date>
15. <dc:type>info:eu-repo/semantics/conferenceObject</dc:type>
16. <dc:type>info:eu-repo/semantics/acceptedVersion</dc:type>
17. <dc:rights>by-nc-nd</dc:rights>
18. <dc:rights>http://creativecommons.org/licenses/by-nc-nd/3.0/es/</dc:rights>
19. <dc:relation>info:eu-repo/grantAgreement/ES/MICINN/CTQ2013-48767-C3-2-R</dc:relation>
20. <dc:identifier>10.1016/j.smallrumres.2017.03.011</dc:identifier>
</oai_dc:dc>

marcxml

Descargar XML

<?xml version="1.0" encoding="UTF-8" ?>

<marc:record type="Bibliographic" schemaLocation="http://www.loc.gov/MARC21/slim http://www.loc.gov/standards/marcxml/schema/MARC21slim.xsd">
1. <marc:leader>00000coc 2200000uu 4500</marc:leader>
2. <marc:controlfield tag="001">70912</marc:controlfield>
3. <marc:controlfield tag="005">20190709135532.0</marc:controlfield>
4. <marc:datafield ind1="7" ind2=" " tag="024">
  1. <marc:subfield code="2">doi</marc:subfield>
  2. <marc:subfield code="a">10.1016/j.smallrumres.2017.03.011</marc:subfield>
  </marc:datafield>
5. <marc:datafield ind1="8" ind2=" " tag="024">
  1. <marc:subfield code="2">sideral</marc:subfield>
  2. <marc:subfield code="a">98577</marc:subfield>
  </marc:datafield>
6. <marc:datafield ind1=" " ind2=" " tag="037">
  1. <marc:subfield code="a">ART-2017-98577</marc:subfield>
  </marc:datafield>
7. <marc:datafield ind1=" " ind2=" " tag="041">
  1. <marc:subfield code="a">eng</marc:subfield>
  </marc:datafield>
8. <marc:datafield ind1=" " ind2=" " tag="100">
  1. <marc:subfield code="a">Pérez-Laguna, V.</marc:subfield>
  </marc:datafield>
9. <marc:datafield ind1=" " ind2=" " tag="245">
  1. <marc:subfield code="a">Daylight photodynamic therapy using methylene blue to treat sheep with dermatophytosis caused by Arthroderma vanbreuseghemii</marc:subfield>
  </marc:datafield>
10. <marc:datafield ind1=" " ind2=" " tag="260">
  1. <marc:subfield code="c">2017</marc:subfield>
  </marc:datafield>
11. <marc:datafield ind1="0" ind2=" " tag="506">
  1. <marc:subfield code="a">Access copy available to the general public</marc:subfield>
  2. <marc:subfield code="f">Unrestricted</marc:subfield>
  </marc:datafield>
12. <marc:datafield ind1="3" ind2=" " tag="520">
  1. <marc:subfield code="a">Arthroderma vanbreuseghemii has been identified molecularly as the causative agent of dermatophytosis in a flock of sheep. It is necessary to explore new treatment alternatives because antifungals are not approved for use on small ruminant animals in the European Union. Antimicrobial photodynamic therapy (aPDT) has been shown to be effective for the treatment of dermatophytosis in humans. It is based on the application of a photosensitizer such as methylene blue (MB) that is activated by visible light to generate reactive oxygen species that are cytotoxic to cells. The use of daylight to perform aPDT (aDL-PDT) avoids the requirement of specific equipment because it uses sunlight to activate the photosensitizer. The aim of our study is to determine the efficacy of aDL-PDT using a 1% MB solution to treat dermatophytosis caused by A. vanbreuseghemii in ewes. Two different topical protocols (1% MB solution spray applications once or twice a week) were assayed in two groups of five infected animals. Twenty-five infected sheep were untreated. All the sheep were exposed to sunlight every day for an approximate duration of 10 h for a total of four weeks. At the end of the study, all the animals treated with aDL-PDT showed the same clinical response to both protocols. In contrast, the animals exposed only to sunlight required an additional two to four weeks before their infections resolved. Conclusion: aDL-PDT with 1% MB solution demonstrates efficacy, safety and efficiency in the treatment of dermatophytosis in sheep.</marc:subfield>
  </marc:datafield>
13. <marc:datafield ind1=" " ind2=" " tag="536">
  1. <marc:subfield code="9">info:eu-repo/grantAgreement/ES/MICINN/CTQ2013-48767-C3-2-R</marc:subfield>
  </marc:datafield>
14. <marc:datafield ind1=" " ind2=" " tag="540">
  1. <marc:subfield code="9">info:eu-repo/semantics/openAccess</marc:subfield>
  2. <marc:subfield code="a">by-nc-nd</marc:subfield>
  3. <marc:subfield code="u">http://creativecommons.org/licenses/by-nc-nd/3.0/es/</marc:subfield>
  </marc:datafield>
15. <marc:datafield ind1=" " ind2=" " tag="590">
  1. <marc:subfield code="a">0.974</marc:subfield>
  2. <marc:subfield code="b">2017</marc:subfield>
  </marc:datafield>
16. <marc:datafield ind1=" " ind2=" " tag="591">
  1. <marc:subfield code="a">AGRICULTURE, DAIRY & ANIMAL SCIENCE</marc:subfield>
  2. <marc:subfield code="b">31 / 59 = 0.525</marc:subfield>
  3. <marc:subfield code="c">2017</marc:subfield>
  4. <marc:subfield code="d">Q3</marc:subfield>
  5. <marc:subfield code="e">T2</marc:subfield>
  </marc:datafield>
17. <marc:datafield ind1=" " ind2=" " tag="592">
  1. <marc:subfield code="a">0.485</marc:subfield>
  2. <marc:subfield code="b">2017</marc:subfield>
  </marc:datafield>
18. <marc:datafield ind1=" " ind2=" " tag="593">
  1. <marc:subfield code="a">Food Animals</marc:subfield>
  2. <marc:subfield code="c">2017</marc:subfield>
  3. <marc:subfield code="d">Q2</marc:subfield>
  </marc:datafield>
19. <marc:datafield ind1=" " ind2=" " tag="593">
  1. <marc:subfield code="a">Animal Science and Zoology</marc:subfield>
  2. <marc:subfield code="c">2017</marc:subfield>
  3. <marc:subfield code="d">Q2</marc:subfield>
  </marc:datafield>
20. <marc:datafield ind1=" " ind2="4" tag="655">
  1. <marc:subfield code="a">info:eu-repo/semantics/conferenceObject</marc:subfield>
  2. <marc:subfield code="v">info:eu-repo/semantics/acceptedVersion</marc:subfield>
  </marc:datafield>
21. <marc:datafield ind1=" " ind2=" " tag="700">
  1. <marc:subfield code="0">(orcid)0000-0001-7294-245X</marc:subfield>
  2. <marc:subfield code="a">Rezusta, A.</marc:subfield>
  3. <marc:subfield code="u">Universidad de Zaragoza</marc:subfield>
  </marc:datafield>
22. <marc:datafield ind1=" " ind2=" " tag="700">
  1. <marc:subfield code="0">(orcid)0000-0002-2724-2785</marc:subfield>
  2. <marc:subfield code="a">Ramos, J. J.</marc:subfield>
  3. <marc:subfield code="u">Universidad de Zaragoza</marc:subfield>
  </marc:datafield>
23. <marc:datafield ind1=" " ind2=" " tag="700">
  1. <marc:subfield code="0">(orcid)0000-0003-0042-8800</marc:subfield>
  2. <marc:subfield code="a">Ferrer, L. M.</marc:subfield>
  3. <marc:subfield code="u">Universidad de Zaragoza</marc:subfield>
  </marc:datafield>
24. <marc:datafield ind1=" " ind2=" " tag="700">
  1. <marc:subfield code="a">Gené, J.</marc:subfield>
  </marc:datafield>
25. <marc:datafield ind1=" " ind2=" " tag="700">
  1. <marc:subfield code="0">(orcid)0000-0003-3570-5791</marc:subfield>
  2. <marc:subfield code="a">Revillo, M. J.</marc:subfield>
  </marc:datafield>
26. <marc:datafield ind1=" " ind2=" " tag="700">
  1. <marc:subfield code="a">Gilaberte, Y.</marc:subfield>
  </marc:datafield>
27. <marc:datafield ind1="2" ind2=" " tag="710">
  1. <marc:subfield code="1">1008</marc:subfield>
  2. <marc:subfield code="2">630</marc:subfield>
  3. <marc:subfield code="a">Universidad de Zaragoza</marc:subfield>
  4. <marc:subfield code="b">Dpto. Microb.Med.Pr.,Sal.Públ.</marc:subfield>
  5. <marc:subfield code="c">Área Microbiología</marc:subfield>
  </marc:datafield>
28. <marc:datafield ind1="2" ind2=" " tag="710">
  1. <marc:subfield code="1">1009</marc:subfield>
  2. <marc:subfield code="2">617</marc:subfield>
  3. <marc:subfield code="a">Universidad de Zaragoza</marc:subfield>
  4. <marc:subfield code="b">Dpto. Patología Animal</marc:subfield>
  5. <marc:subfield code="c">Área Medicina y Cirugía Animal</marc:subfield>
  </marc:datafield>
29. <marc:datafield ind1=" " ind2=" " tag="773">
  1. <marc:subfield code="g">150 (2017), 97-101</marc:subfield>
  2. <marc:subfield code="p">Small rumin. res.</marc:subfield>
  3. <marc:subfield code="t">Small Ruminant Research</marc:subfield>
  4. <marc:subfield code="x">0921-4488</marc:subfield>
  </marc:datafield>
30. <marc:datafield ind1="4" ind2=" " tag="856">
  1. <marc:subfield code="s">715538</marc:subfield>
  2. <marc:subfield code="u">http://zaguan.unizar.es/record/70912/files/texto_completo.pdf</marc:subfield>
  3. <marc:subfield code="y">Postprint</marc:subfield>
  </marc:datafield>
31. <marc:datafield ind1="4" ind2=" " tag="856">
  1. <marc:subfield code="s">47677</marc:subfield>
  2. <marc:subfield code="u">http://zaguan.unizar.es/record/70912/files/texto_completo.jpg?subformat=icon</marc:subfield>
  3. <marc:subfield code="x">icon</marc:subfield>
  4. <marc:subfield code="y">Postprint</marc:subfield>
  </marc:datafield>
32. <marc:datafield ind1="C" ind2="O" tag="909">
  1. <marc:subfield code="o">oai:zaguan.unizar.es:70912</marc:subfield>
  2. <marc:subfield code="p">articulos</marc:subfield>
  3. <marc:subfield code="p">driver</marc:subfield>
  </marc:datafield>
33. <marc:datafield ind1=" " ind2=" " tag="951">
  1. <marc:subfield code="a">2019-07-09-12:03:03</marc:subfield>
  </marc:datafield>
34. <marc:datafield ind1=" " ind2=" " tag="980">
  1. <marc:subfield code="a">ARTICLE</marc:subfield>
  </marc:datafield>
</marc:record>