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A disulfide bond A-like oxidoreductase is a strong candidate gene for self-incompatibility in apricot (Prunus armeniaca) pollen

Identificadores del recurso

Munoz-Sanz, J. V., Zuriaga, E., Badenes, M. L. & Romero, C. (2017). A disulfide bond A-like oxidoreductase is a strong candidate gene for self-incompatibility in apricot (prunus armeniaca) pollen. Journal of Experimental Botany, 68(18), 5069-5078.

0022-0957

http://hdl.handle.net/20.500.11939/6076

10.1093/jxb/erx336

Origin

(ReDIVIA: Repositori Digital de l'Institut Valencià d'Investigacions Agràries)

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Title:: A disulfide bond A-like oxidoreductase is a strong candidate gene for self-incompatibility in apricot (Prunus armeniaca) pollen
Description:: S-RNase based gametophytic self-incompatibility (SI) is a widespread prezygotic reproductive barrier in flowering plants. In the Solanaceae, Plantaginaceae and Rosaceae gametophytic SI is controlled by the pistil-specific S-RNases and the pollen S-locus F-box proteins but non-S-specific factors, namely modifiers, are also required. In apricot, Prunus armeniaca (Rosaceae), we previously mapped two pollen-part mutations that confer self-compatibility in cultivars Canino and Katy at the distal end of chromosome 3 (M-locus) unlinked to the S-locus. Here, we used high-resolution mapping to identify the M-locus with an similar to 134 kb segment containing ParM-1-16 genes. Gene expression analysis identified four genes preferentially expressed in anthers as modifier gene candidates, ParM-6, -7, -9 and -14. Variant calling of WGS Illumina data from Canino, Katy, and 10 self-incompatible cultivars detected a 358 bp miniature inverted-repeat transposable element (MITE) insertion in ParM-7 shared only by self-compatible apricots, supporting ParM-7 as strong candidate gene required for SI. ParM-7 encodes a disulfide bond A-like oxidoreductase protein, which we named ParMDO. The MITE insertion truncates the ParMDO ORF and produces a loss of SI function, suggesting that pollen rejection in Prunus is dependent on redox regulation. Based on phylogentic analyses we also suggest that ParMDO may have originated from a tandem duplication followed by subfunctionalization and pollenspecific expression.
Source:: electronico
Idioma:
Autor/Productor:: Munoz-Sanz, Juan V.; Zuriaga, Elena; Badenes, María L.; Romero, Carlos
Rights:: Atribución-NoComercial-SinDerivadas 3.0 España; http://creativecommons.org/licenses/by-nc-nd/3.0/es/
Date:: 2018-05-09T16:31:00Z; 2017
Tipo de recurso:: article; publishedVersion

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1. <dc:title>A disulfide bond A-like oxidoreductase is a strong candidate gene for self-incompatibility in apricot (Prunus armeniaca) pollen</dc:title>
2. <dc:creator>Munoz-Sanz, Juan V.</dc:creator>
3. <dc:creator>Zuriaga, Elena</dc:creator>
4. <dc:creator>Badenes, María L.</dc:creator>
5. <dc:creator>Romero, Carlos</dc:creator>
6. <dc:description>S-RNase based gametophytic self-incompatibility (SI) is a widespread prezygotic reproductive barrier in flowering plants. In the Solanaceae, Plantaginaceae and Rosaceae gametophytic SI is controlled by the pistil-specific S-RNases and the pollen S-locus F-box proteins but non-S-specific factors, namely modifiers, are also required. In apricot, Prunus armeniaca (Rosaceae), we previously mapped two pollen-part mutations that confer self-compatibility in cultivars Canino and Katy at the distal end of chromosome 3 (M-locus) unlinked to the S-locus. Here, we used high-resolution mapping to identify the M-locus with an similar to 134 kb segment containing ParM-1-16 genes. Gene expression analysis identified four genes preferentially expressed in anthers as modifier gene candidates, ParM-6, -7, -9 and -14. Variant calling of WGS Illumina data from Canino, Katy, and 10 self-incompatible cultivars detected a 358 bp miniature inverted-repeat transposable element (MITE) insertion in ParM-7 shared only by self-compatible apricots, supporting ParM-7 as strong candidate gene required for SI. ParM-7 encodes a disulfide bond A-like oxidoreductase protein, which we named ParMDO. The MITE insertion truncates the ParMDO ORF and produces a loss of SI function, suggesting that pollen rejection in Prunus is dependent on redox regulation. Based on phylogentic analyses we also suggest that ParMDO may have originated from a tandem duplication followed by subfunctionalization and pollenspecific expression.</dc:description>
7. <dc:date>2018-05-09T16:31:00Z</dc:date>
8. <dc:date>2018-05-09T16:31:00Z</dc:date>
9. <dc:date>2017</dc:date>
10. <dc:type>article</dc:type>
11. <dc:type>publishedVersion</dc:type>
12. <dc:identifier>Munoz-Sanz, J. V., Zuriaga, E., Badenes, M. L. & Romero, C. (2017). A disulfide bond A-like oxidoreductase is a strong candidate gene for self-incompatibility in apricot (prunus armeniaca) pollen. Journal of Experimental Botany, 68(18), 5069-5078.</dc:identifier>
13. <dc:identifier>0022-0957</dc:identifier>
14. <dc:identifier>http://hdl.handle.net/20.500.11939/6076</dc:identifier>
15. <dc:identifier>10.1093/jxb/erx336</dc:identifier>
16. <dc:identifier />
17. <dc:language>en</dc:language>
18. <dc:rights>Atribución-NoComercial-SinDerivadas 3.0 España</dc:rights>
19. <dc:rights>http://creativecommons.org/licenses/by-nc-nd/3.0/es/</dc:rights>
20. <dc:source>electronico</dc:source>
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