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A broad exploration of a transgenic population of citrus: stability of gene expression and phenotype

Identificadores del recurso

Cervera, M., Pina, JA, Juarez, J., Navarro, L., Pena, L. (2000). A broad exploration of a transgenic population of citrus: stability of gene expression and phenotype. Theoretical and Applied Genetics, 100(5), 670-677.

0040-5752

http://hdl.handle.net/20.500.11939/5013

10.1007/s001220051338

Origin

(ReDIVIA: Repositori Digital de l'Institut Valencià d'Investigacions Agràries)

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Title:: A broad exploration of a transgenic population of citrus: stability of gene expression and phenotype
Description:: A collection of 70 transgenic citrus plants for the uidA and nptII genes have been maintained under screenhouse conditions over a period of 4-5 years. A detailed scanning of the plants allowed us to detect four phenotypic off-type plants and a large variation of transgene integration and expression patterns among the population. Off-type plants were analysed and characterised as nucellar tetraploids, probably originating from tetraploid starting tissues rather than from somaclonal variation events. Transgene integration and expression analyses revealed that: (I) a significant negative correlation was found between copy number and GUS activity; (2) rearrangements of the T-DNA inserts did not imply low expression levels; and (3) stability of integration and expression of the transgenes was confirmed for all the transformants grown under natural environmental conditions. These combined features validate transformation as a tool for the genetic improvement of citrus.
Source:: Impreso
Idioma:
Autor/Productor:: Cervera, Magdalena; Pina, José A.; Juárez, José; Navarro, Luis; Pena, Leandro
Rights:: openAccess
Date:: 2017-06-01T10:11:33Z; 2000; MAR
Tipo de recurso:: article

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1. <dc:title>A broad exploration of a transgenic population of citrus: stability of gene expression and phenotype</dc:title>
2. <dc:creator>Cervera, Magdalena</dc:creator>
3. <dc:creator>Pina, José A.</dc:creator>
4. <dc:creator>Juárez, José</dc:creator>
5. <dc:creator>Navarro, Luis</dc:creator>
6. <dc:creator>Pena, Leandro</dc:creator>
7. <dc:description>A collection of 70 transgenic citrus plants for the uidA and nptII genes have been maintained under screenhouse conditions over a period of 4-5 years. A detailed scanning of the plants allowed us to detect four phenotypic off-type plants and a large variation of transgene integration and expression patterns among the population. Off-type plants were analysed and characterised as nucellar tetraploids, probably originating from tetraploid starting tissues rather than from somaclonal variation events. Transgene integration and expression analyses revealed that: (I) a significant negative correlation was found between copy number and GUS activity; (2) rearrangements of the T-DNA inserts did not imply low expression levels; and (3) stability of integration and expression of the transgenes was confirmed for all the transformants grown under natural environmental conditions. These combined features validate transformation as a tool for the genetic improvement of citrus.</dc:description>
8. <dc:date>2017-06-01T10:11:33Z</dc:date>
9. <dc:date>2017-06-01T10:11:33Z</dc:date>
10. <dc:date>2000</dc:date>
11. <dc:date>MAR</dc:date>
12. <dc:type>article</dc:type>
13. <dc:identifier>Cervera, M., Pina, JA, Juarez, J., Navarro, L., Pena, L. (2000). A broad exploration of a transgenic population of citrus: stability of gene expression and phenotype. Theoretical and Applied Genetics, 100(5), 670-677.</dc:identifier>
14. <dc:identifier>0040-5752</dc:identifier>
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