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α-Lipoic acid reduces fatty acid esterification and lipogenesis in adipocytes from overweight/obese subjects

Identificadores del recurso

https://investigacion.unirioja.es/documentos/5d31843f2999521056384b7e

info:doi:10.1002/OBY.20846

urn:issn:1930-739X

urn:issn:1930-7381

Origin

(Repositorio Institucional de la Universidad de La Rioja (RIUR))

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Title:: α-Lipoic acid reduces fatty acid esterification and lipogenesis in adipocytes from overweight/obese subjects
Description:: Objective: -Lipoic acid (-LA) is a natural occurring antioxidant with beneficial effects on obesity. The aim of this study was to investigate the putative effects of -LA on triglyceride accumulation and lipogenesis in subcutaneous adipocytes from overweight/obese subjects and to determine the potential mechanisms involved. Methods: Fully differentiated human subcutaneous adipocytes were treated with -LA (100 and 250 M) during 24 h for studying triglyceride content, de novo lipogenesis, and levels of key lipogenic enzymes. The involvement of AMP-activated protein kinase (AMPK) activation was also evaluated. Results: -LA down-regulated triglyceride content by inhibiting fatty acid esterification and de novo lipogenesis. These effects were mediated by reduction in fatty acid synthase (FAS), stearoyl-coenzyme A desaturase 1, and diacylglycerol O-acyltransferase 1 protein levels. Interestingly, -LA increased AMPK and acetyl CoA carboxylase phosphorylation, while the presence of the AMPK inhibitor Compound C reversed the inhibition observed on FAS protein levels. Conclusions: -LA down-regulates key lipogenic enzymes, inhibiting lipogenesis and reducing triglyceride accumulation through the activation of AMPK signaling pathway in human subcutaneous adipocytes from overweight/obese subjects.
Idioma:: English
Relation:: info:eu-repo/semantics/altIdentifier/doi/10.1002/OBY.20846; info:eu-repo/semantics/altIdentifier/pmid/25045030; info:eu-repo/semantics/altIdentifier/eissn/1930-739X; info:eu-repo/semantics/altIdentifier/pissn/1930-7381; α-Lipoic acid reduces fatty acid esterification and lipogenesis in adipocytes from overweight/obese subjects, 2014, vol. 22, núm. 10, pág. 2210-2215
Autor/Productor:: Fernández-Galilea, Marta; Pérez-Matute, Patricia; Prieto-Hontoria, Pedro L.; Sáinz, Neira; López-Yoldi, Miguel; Houssier, Marianne; Martínez, J. Alfredo; Langin, Dominique; Moreno-Aliaga, María J.
Publisher:: Blackwell Publishing Inc.
Rights:: info:eu-repo/semantics/closedAccess
Date:: 2014
Tipo de recurso:: info:eu-repo/semantics/article; Subtype: Article; info:eu-repo/semantics/publishedVersion
Format:: application/pdf

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1. <dc:title>α-Lipoic acid reduces fatty acid esterification and lipogenesis in adipocytes from overweight/obese subjects</dc:title>
2. <dc:creator>Fernández-Galilea, Marta</dc:creator>
3. <dc:creator>Pérez-Matute, Patricia</dc:creator>
4. <dc:creator>Prieto-Hontoria, Pedro L.</dc:creator>
5. <dc:creator>Sáinz, Neira</dc:creator>
6. <dc:creator>López-Yoldi, Miguel</dc:creator>
7. <dc:creator>Houssier, Marianne</dc:creator>
8. <dc:creator>Martínez, J. Alfredo</dc:creator>
9. <dc:creator>Langin, Dominique</dc:creator>
10. <dc:creator>Moreno-Aliaga, María J.</dc:creator>
11. <dc:description>Objective: -Lipoic acid (-LA) is a natural occurring antioxidant with beneficial effects on obesity. The aim of this study was to investigate the putative effects of -LA on triglyceride accumulation and lipogenesis in subcutaneous adipocytes from overweight/obese subjects and to determine the potential mechanisms involved. Methods: Fully differentiated human subcutaneous adipocytes were treated with -LA (100 and 250 M) during 24 h for studying triglyceride content, de novo lipogenesis, and levels of key lipogenic enzymes. The involvement of AMP-activated protein kinase (AMPK) activation was also evaluated. Results: -LA down-regulated triglyceride content by inhibiting fatty acid esterification and de novo lipogenesis. These effects were mediated by reduction in fatty acid synthase (FAS), stearoyl-coenzyme A desaturase 1, and diacylglycerol O-acyltransferase 1 protein levels. Interestingly, -LA increased AMPK and acetyl CoA carboxylase phosphorylation, while the presence of the AMPK inhibitor Compound C reversed the inhibition observed on FAS protein levels. Conclusions: -LA down-regulates key lipogenic enzymes, inhibiting lipogenesis and reducing triglyceride accumulation through the activation of AMPK signaling pathway in human subcutaneous adipocytes from overweight/obese subjects.</dc:description>
12. <dc:date>2014</dc:date>
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20. <dc:language>eng</dc:language>
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