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9-cis-retinoic acid during in vitro maturation improves development of the bovine oocyte and increases midkine but not IGF-I expression in cumulus-granulosa cells

Identificadores del recurso

Gómez, Enrique; Royo, Luis-José; Duque, Paloma; Carneiro, Gustavo; Hidalgo, Carlos; Goyache, Félix [et. al]. 9-cis-retinoic acid during in vitro maturation improves development of the bovine oocyte and increases midkine but not IGF-I expression in cumulus-granulosa cells. Molecular Reproduction and Development. 2003; 66 (3): 247-255.

1040-452X

http://ria.asturias.es/RIA/handle/123456789/2088

Procedència

(Repositorio Institucional de Asturias: RIA)

Fitxa

Títol:: 9-cis-retinoic acid during in vitro maturation improves development of the bovine oocyte and increases midkine but not IGF-I expression in cumulus-granulosa cells
Tema:: Embryo; Ethanol; Viability; Cytoplasmic granule; Publicado
Descripció:: The isomer 9-cis of retinoic acid (9-cis-RA) exerts a beneficial effect on bovine in vitro development when added to in vitro maturation (IVM) culture. In the present work, 9-cis-RA 5 nM was found to be stimulatory as opposed to 500 nM (toxic). Cumulus-oocyte complexes (COCs) were treated with the found physiological dose 9-cis-RA 5 nM, and the next determinations performed: (1) relative expression of midkine (MK) and IGF-I, by reverse transcriptase-polymerase chain reaction (RT-PCR), in cumulus-granulosa cells detached from oocytes; (2) cytoplasmic granular migration, by labeling of oocytes with fluoroscein isothiocyanate lectins; and (3) in vitro survival of blastocysts after vitrification and warming. Gene expression of MK was enhanced by 9-cis-RA, but not by 1% ethanol (vehicle). However, we did not detect IGF-I expression, both in dependence on or in the absence of 9-cis-RA acting on cumulus-granulosa cells. The ability of vitrified blastocysts to survive in vitro was not improved by 9-cis-RA. Nevertheless, since only blastocysts obtained from oocytes matured with serum survived, more factors should be considered when evaluating cryopreservation survival. The complete granular migration observed in oocytes matured with 9-cis-RA anticipates the gain in developmental competence of the oocyte, being MK probably involved in this beneficial effect.
Font:: 247;255
Idioma:: English
Relació:: Molecular Reproduction and Development; 66; 3
Autor/Productor:: Gómez, Enrique; Royo Martín, Luis J.; Duque, Paloma; Carneiro, Gustavo; Hidalgo Ordóñez, Carlos O.; Goyache, Félix; Lorenzo, Pedro L.; Álvarez, Isabel; Facal, Nieves; Díez, Carmen
Editor:: WILEY-LISS, INC
Drets:: http://creativecommons.org/licenses/by-nc-sa/3.0/deed.es
Data:: 2012-11-07T13:40:11Z; 2003-11
Tipo de recurso:: article
Format:: application/pdf

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    1. <element name="author">
      1. <element name="none">
        <field name="value">Gómez, Enrique</field>
        <field name="value">Royo Martín, Luis J.</field>
        <field name="value">Duque, Paloma</field>
        <field name="value">Carneiro, Gustavo</field>
        <field name="value">Hidalgo Ordóñez, Carlos O.</field>
        <field name="value">Goyache, Félix</field>
        <field name="value">Lorenzo, Pedro L.</field>
        <field name="value">Álvarez, Isabel</field>
        <field name="value">Facal, Nieves</field>
        <field name="value">Díez, Carmen</field>
        </element>
      </element>
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  2. <element name="date">
    1. <element name="accessioned">
      1. <element name="none">
        <field name="value">2012-11-07T13:40:11Z</field>
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    2. <element name="available">
      1. <element name="none">
        <field name="value">2012-11-07T13:40:11Z</field>
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    3. <element name="issued">
      1. <element name="none">
        <field name="value">2003-11</field>
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  3. <element name="identifier">
    1. <element name="citation">
      1. <element name="eng">
        <field name="value">Gómez, Enrique; Royo, Luis-José; Duque, Paloma; Carneiro, Gustavo; Hidalgo, Carlos; Goyache, Félix [et. al]. 9-cis-retinoic acid during in vitro maturation improves development of the bovine oocyte and increases midkine but not IGF-I expression in cumulus-granulosa cells. Molecular Reproduction and Development. 2003; 66 (3): 247-255.</field>
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    2. <element name="issn">
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        <field name="value">1040-452X</field>
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    3. <element name="uri">
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        <field name="value">http://ria.asturias.es/RIA/handle/123456789/2088</field>
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  4. <element name="description">
    1. <element name="abstract">
      1. <element name="eng">
        <field name="value">The isomer 9-cis of retinoic acid (9-cis-RA) exerts a beneficial effect on bovine in vitro development when added to in vitro maturation (IVM) culture. In the present work, 9-cis-RA 5 nM was found to be stimulatory as opposed to 500 nM (toxic). Cumulus-oocyte complexes (COCs) were treated with the found physiological dose 9-cis-RA 5 nM, and the next determinations performed: (1) relative expression of midkine (MK) and IGF-I, by reverse transcriptase-polymerase chain reaction (RT-PCR), in cumulus-granulosa cells detached from oocytes; (2) cytoplasmic granular migration, by labeling of oocytes with fluoroscein isothiocyanate lectins; and (3) in vitro survival of blastocysts after vitrification and warming. Gene expression of MK was enhanced by 9-cis-RA, but not by 1% ethanol (vehicle). However, we did not detect IGF-I expression, both in dependence on or in the absence of 9-cis-RA acting on cumulus-granulosa cells. The ability of vitrified blastocysts to survive in vitro was not improved by 9-cis-RA. Nevertheless, since only blastocysts obtained from oocytes matured with serum survived, more factors should be considered when evaluating cryopreservation survival. The complete granular migration observed in oocytes matured with 9-cis-RA anticipates the gain in developmental competence of the oocyte, being MK probably involved in this beneficial effect.</field>
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    1. <element name="iso">
      1. <element name="eng">
        <field name="value">eng</field>
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  6. <element name="publisher">
    1. <element name="eng">
      1. <field name="value">WILEY-LISS, INC</field>
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  7. <element name="relation">
    1. <element name="ispartof">
      1. <element name="eng">
        <field name="value">Molecular Reproduction and Development</field>
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    2. <element name="haspart">
      1. <element name="eng">
        <field name="value">66</field>
        </element>
      </element>
    3. <element name="hasversion">
      1. <element name="eng">
        <field name="value">3</field>
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  8. <element name="rights">
    1. <element name="eng">
      1. <field name="value">http://creativecommons.org/licenses/by-nc-sa/3.0/deed.es</field>
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  9. <element name="source">
    1. <element name="none">
      1. <field name="value">247;255</field>
      </element>
    </element>
  10. <element name="subject">
    1. <element name="eng">
      1. <field name="value">Embryo</field>
      2. <field name="value">Ethanol</field>
      3. <field name="value">Viability</field>
      4. <field name="value">Cytoplasmic granule</field>
      </element>
    2. <element name="classification">
      1. <element name="eng">
        <field name="value">Publicado</field>
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      </element>
    </element>
  11. <element name="title">
    1. <element name="eng">
      1. <field name="value">9-cis-retinoic acid during in vitro maturation improves development of the bovine oocyte and increases midkine but not IGF-I expression in cumulus-granulosa cells</field>
      </element>
    </element>
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      1. <field name="value">article</field>
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  3. <field name="lastModifyDate">2015-11-04 12:36:55.264</field>
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  1. <field name="name">RIA</field>
  2. <field name="mail">gemma.gonzalez@ricoh.es</field>
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