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<dc:title lang="en-US">β-Galactosidase activity of Escherichia coli under long-term starvation, alterations in temperature, and different nutrient conditions in lake water</dc:title>
<dc:creator>R. Özkanca; Ondokuz Mayıs University, Faculty of Science and Arts, Department of Biological Sciences, Kurupelit, Samsun, Turkey</dc:creator>
<dc:subject lang="en-US">Escherichia coli; β-galactosidase activity; long-term starvation; nutrient sources effect</dc:subject>
<dc:description lang="en-US">β-Galactosidase activity of Escherichia coli was investigated in response to long-term starvation, changes in temperature and the presence of certain nutrient sources in lake water. β-Galactosidase activity decreased markedly in filtered-autoclaved lake water at 25°C and 37°C, whereas it remained almost constant at 4°C and 15°C for 60 days. Increases in β-galactosidase activity were observed in response to the following nutrient sources: glycine, serine, methionine and ammonium sulfate at 4°C; glycine and ammonium sulfate at 15°C; glycine, serine, methionine and ammonium sulfate at 30°C. Glycine addition led to an increase in β-galactosidase activity of almost five and seven orders of magnitude at 15°C and 30°C, respectively. In addition, L-methionine had the strongest influence on b-galactosidase activity, which was detected as an increase of seven and eleven ordersof magnitude at 4°C and 30°C, respectively. The effect of several amino acids and other nitrogen sources depended on the concentration of the nutrient source and the temperature. The results showed that, in lake water, long-term starvation, temperature change, and variationsin nitrogen sourcesalter β-galactosidase activity. Those effects should be taken into account when monitoring coliformsfrom the environment.</dc:description>
<dc:publisher lang="ca-ES">International Microbiology</dc:publisher>
<dc:publisher lang="en-US">International Microbiology</dc:publisher>
<dc:date>2010-09-21</dc:date>
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<dc:identifier>http://revistes.iec.cat/index.php/IM/article/view/11061</dc:identifier>
<dc:source lang="ca-ES">International Microbiology; Vol. 5, Núm. 3 (2002); 121-125</dc:source>
<dc:source lang="en-US">International Microbiology; Vol. 5, Núm. 3 (2002); 121-125</dc:source>
<dc:language>en</dc:language>
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<title>β-Galactosidase activity of Escherichia coli under long-term starvation, alterations in temperature, and different nutrient conditions in lake water</title>
<author>R. Özkanca; Ondokuz Mayıs University, Faculty of Science and Arts, Department of Biological Sciences, Kurupelit, Samsun, Turkey</author>
<date>2010-09-21</date>
<copyright>Submission of a manuscript to International Microbiology implies: that the work described has not been published before, including publication in the World Wide Web (except in the form of an Abstract or as part of a published lecture, review, or thesis); that it is not under consideration for publication elsewhere; that all the coauthors have agreed to its publication. The corresponding author signs for and accepts responsability for releasing this material and will act on behalf of any and all coauthors regarding the editorial review and publication process.<br /><br />If an article is accepted for publication in International Microbiology, the authors (or other copyright holder) must transfer to the journal the right–not exclusive–to reproduce and distribute the article including reprints, translations, photographic reproductions, microform, electronic form (offline, online) or any other reproductions of similar nature. Nevertheless, all article in International Microbiology will be available on the Internet to any reader at no cost. The journal allows users to freely download, copy, print, distribute, search, and link to the full text of any article, provided the authorship and source of the published article is cited. The copyright owner's consent does not include copying for new works, or resale. In these cases, the specific written permission of International Microbiology must first be obtained.<br /><br />Authors are requested to create a link to the published article on the journal's website. The link must be accompanied by the following text: "The original publication is available on LINK at <<a title="IM" href="http://www.im.microbios.org" target="_blank">http://www.im.microbios.org</a>>. Please use the appropiate URL for the article in LINK. Articles disseminated via LINK are indexed, abstracted, and referenced by many abstracting and information services, bibliographic networks, subscription agencies, library networks, and consortia.</copyright>
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<keyword>Escherichia coli; β-galactosidase activity; long-term starvation; nutrient sources effect</keyword>
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<abstract>β-Galactosidase activity of Escherichia coli was investigated in response to long-term starvation, changes in temperature and the presence of certain nutrient sources in lake water. β-Galactosidase activity decreased markedly in filtered-autoclaved lake water at 25°C and 37°C, whereas it remained almost constant at 4°C and 15°C for 60 days. Increases in β-galactosidase activity were observed in response to the following nutrient sources: glycine, serine, methionine and ammonium sulfate at 4°C; glycine and ammonium sulfate at 15°C; glycine, serine, methionine and ammonium sulfate at 30°C. Glycine addition led to an increase in β-galactosidase activity of almost five and seven orders of magnitude at 15°C and 30°C, respectively. In addition, L-methionine had the strongest influence on b-galactosidase activity, which was detected as an increase of seven and eleven ordersof magnitude at 4°C and 30°C, respectively. The effect of several amino acids and other nitrogen sources depended on the concentration of the nutrient source and the temperature. The results showed that, in lake water, long-term starvation, temperature change, and variationsin nitrogen sourcesalter β-galactosidase activity. Those effects should be taken into account when monitoring coliformsfrom the environment.</abstract>
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